Heirarchical assembly methods for genome engineering
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United States of America Patent
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Issued Date -
Jan 4, 2007
app pub date -
Jan 12, 2006
filing date -
Jun 30, 2005
priority date (Note) -
Abandoned
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Abstract
The present invention provides recombination based methods for assembling nucleic acids. In certain aspects the present invention provides hierarchical assembly methods for producing genome sized polynucleotide constructs. The methods may be used for assembling large polynucleotide constructs, for synthesizing synthetic genomes, or for introducing a plurality of nucleotide changes throughout the genome of an organism. In another aspect, the invention provides cells having increased genomic stability. For example, cells comprising alterations in at least a substantial portion of the transposons in the genome are provided.
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