Plating media for the identification of Yersinia pestis
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United States of America Patent
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Issued Date -
Jan 4, 2007
app pub date -
Jun 28, 2005
filing date -
Jun 28, 2004
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Abstract
A plating media for identifying Yersinia pestis bacteria having nutrients for promoting growth of Yersinia pestis thereby producing beta-glucosidase, carbohydrate that is incapable of reacting with Yersinia pestis, a pH indicator dye, a chromogenic substrate that reacts to beta-glucosidase to form precipitate, and an agent to solidify the mixture, whereby a microorganism which ferments the carbohydrate but does not produce beta-glucosidase will produce colonies of the color determined by the pH indicator dye, Yersinia pestis and other microorganisms that do not ferment the carbohydrate but produce beta-glucosidase activate the substrate to color their colonies with the color of precipitate released by the substrate, and other bacteria which ferment the carbohydrate and produce beta-glucosidase produce colonies of the color that results from mixing the colors described above.
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