Accessible polynucleotide libraries and methods of use thereof
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United States of America Patent
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Issued Date -
Dec 14, 2006
app pub date -
May 17, 2006
filing date -
May 18, 2005
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Abandoned
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Abstract
Disclosed are methods of assembling nucleic acid constructs from component parts in a manner that is not dependent on the sequence of the component parts. The methods may be used to assemble large nucleic acid constructs from multiple component parts in one or more reactions. The methods may also be used to assemble two or more nucleic acid constructs in the same reaction mixture. In exemplary embodiments, the methods involve formation of a Holliday junction or bridge structure using a junction oligonucleotide.
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