Method for analyzing cell free notch cleavage and method for drug screening
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
N/A
Issued Date -
N/A
app pub date -
May 4, 2006
filing date -
Nov 10, 2003
priority date (Note) -
Abandoned
status (Latency Note)
Importance
Abstract
The present invention provides a method for analyzing cell-free Notch cleavage which can be utilized for drug screening of .gamma.-secretase inhibitors effective for the treatment of Alzheimer's disease and the drug screening method, is the method for analyzing cell-free Notch cleavage having a cell-free cleavage reaction step of contacting a crude membrane fraction of cells in which a Notch protein mutant has been expressed with a subject substance and a detection step of detecting a fragment of an amino terminal side and a fragment of a carboxyl terminal side produced by intramembrane proteolysis (dual sequential cleavage in putative transmembrane domain) of the Notch protein mutant in the above cell free cleavage reaction step, wherein the effect of the subject substance on the intramembrane proteolysis of the Notch protein mutant is analyzed, and the Notch protein mutant has at least the putative transmembrane domain of a Notch protein, deletes a part of an amino acid sequence at the amino terminal side than at least the putative transmembrane domain and has antibody recognition sites at the amino terminal side than the putative transmembrane domain and the carboxyl terminal side, respectively.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully