ISOLATION OF CELLS FROM BONE
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May 25, 2006
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May 26, 2005
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Abstract
The present invention provides a method for isolating cells from bone(s) harvested from a mammal. The method comprising the steps of: (a) obtaining a bone(s) containing bone marrow from the mammal; (b) fragmentation of the bone(s); (c) washing the bone fragments produced from step (b) and recovery of dislodged cells; (d) treating the bone fragments with a composition to release cells adhered to the endosteal region of the bone; (e) harvesting the cells following treatment of the bone fragments according to step (d); and (f) optionally combining the cells from steps (c) and (e).
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