Method for the rapid taxonomic identification of pathogenic microorganisms and their toxic proteins
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United States of America Patent
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Issued Date -
Nov 16, 2006
app pub date -
Nov 14, 2005
filing date -
Nov 12, 2003
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Abandoned
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Abstract
The present invention describes a method for the rapid binding of pathogenic microorganisms and their toxic proteins with ligands that have been covalently tethered at some distance from the surface of a substrate. Ligands directed to microbes are covalently attached to the substrate surface by tethers that are between 35 .ANG. and 50 .ANG. in length for optimal binding efficacy. Ligands directed to capture and concentrate proteinaceous materials are covalently attached to the substrate surface by tethers that are between 35 .ANG. and 50 .ANG. in length for optimum assay kinetics. The ligands described herein include heme compounds, siderophores, polysaccharides, and peptides specific for toxic proteins, outer membrane proteins and conjugated lipids. Non-binding components of the solution to be analyzed are separated from the bound fraction and binding is confirmed by detection of the analyte via microscopy, fluorescence, epifluorescence, luminescence, phosphorescence, radioactivity, or optical absorbance. By patterning numerous ligands in an array on a substrate surface it is possible to taxonomically identify the microorganism by analysis of the binding pattern of the sample to the array.
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