L-glutamate oxidase
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
N/A
Issued Date -
N/A
app pub date -
Apr 7, 2006
filing date -
Apr 19, 2000
priority date (Note) -
Abandoned
status (Latency Note)
Importance
Abstract
The present invention provides a novel L-glutamate oxidase, a gene encoding the enzyme, and a method for producing the enzyme. By use of a gene encoding the enzyme, L-glutamate oxidase can be readily prepared at low costs through a recombinant DNA technique. The novel L-glutamate oxidase has the following physicochemical properties: (A) action: catalyzing the following reaction: L-glutamic acid+O.sub.2+H.sub.2O.fwdarw..alpha.-ketoglutaric acid+H.sub.2O.sub.2+NH.sub.3; (B) substrate specificity: being specific to L-glutamic acid; (C) molecular weight and subunit structure: molecular weight as determined through SDS-polyacrylamide gel electrophoresis of 70,000.+-.6,000, molecular weight as determined through gel filtration of 140,000.+-.10,000, and being a dimer formed of the same subunits having a molecular weight of 70,000.+-.6,000; (D) optimum pH: around pH 6.0 to 8.5; (E) heat stability: being stable up to 60.degree. C. at a pH of 7.4 for 30 minutes; and (F) coenzyme: flavin adenine dinucleotide (FAD).
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully