Fluorescence polarisation
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United States of America Patent
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Issued Date -
Sep 21, 2006
app pub date -
Jul 5, 2004
filing date -
Jul 4, 2003
priority date (Note) -
Abandoned
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Abstract
There is described a method for detecting alkylated cytosine in double stranded DNA employing one or more enzymes that differentially modify alkylated cytosine and cytosine. At least one region of the DNA is converted to single stranded DNA and the enzyme is reacted with a target region in the single stranded DNA. The presence or level of alkylated cytosine in the target region is detected by determining the level of enzymatic modification of the target region by the enzyme.
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