In vitro differentiation and maturation of mouse embryonic stem cells into hepatocytes
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United States of America Patent
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Mar 9, 2006
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Mar 10, 2005
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Abstract
The present invention provides a method for preparing a mature hepatocyte from an embryonic stem cell in vitro, comprising: (a) culturing the embryonic stem cell so as to differentiate into an endodermal cell; (b) isolating the endodermal cell from a population of the differenciated cell; and (c) culturing the isolated endodermal cell in the presence of a Thy 1-positive mesenchymal cell.
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