Methods for assembly of high fidelity synthetic polynucleotides
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United States of America Patent
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Issued Date -
Aug 31, 2006
app pub date -
Feb 28, 2005
filing date -
Feb 28, 2005
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Abandoned
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Abstract
Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of polynucleotide polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre-amplification of one or more oligonucleotides using 'universal' primers, reduction of the error rate in oligonucleotide and/or polynucleotide products, and sequence optimization and oligonucleotides design.
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