Methods for retrotransposing long interspersed elements (lines)
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United States of America Patent
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Issued Date -
Aug 17, 2006
app pub date -
Nov 26, 2002
filing date -
Jan 31, 2002
priority date (Note) -
Abandoned
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Abstract
The present invention provides methods for retrotransposing LINEs. The present invention relates to methods for transcribing RNAs comprising LINE 3'UTR fragments in cells, and retrotransposing these RNAs by using viral vectors to provide their LINE ORF proteins in trans. This invention also relates to methods for altering LINE retrotransposition target sites by replacing a LINE endonuclease domain with an endonuclease domain of another LINE. The methods of LINE retrotransposition of the present invention are useful for novel gene delivery.
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