Engineering redox proteins
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United States of America Patent
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Issued Date -
Jul 6, 2006
app pub date -
Dec 3, 2003
filing date -
Dec 4, 2002
priority date (Note) -
Abandoned
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Abstract
The protein of this invention comprises a 4-.alpha.-helix bundle motif formed from the .alpha.-helices of rop (repressor of primer) and a redox centre. In this invention the redox centre is preferably haem and the iron is preferably coordinated to the .alpha.-helices of the rop structure via histidine residues. Such a protein is very stable and has the same specific activities as natural redox proteins with the increased stability in the interaction with electrode surfaces. Also provided by this invention is a method of engineering proteins to develop redox proteins from structures which previously had no redox functions.
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