Method of screening substance interacting with ABC protein
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United States of America Patent
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app pub date -
Sep 23, 2005
filing date -
Mar 25, 2003
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Abstract
The present invention relates to a method for measuring transporter activity of ABC protein that may measure not only small amount of samples with small data fluctuation and good reproducibility, but also many samples simultaneously and concisely; a screening method for substances that interact with ABC protein using said measuring method; and a kit for measuring transporter activity of ABC protein that may advantageously be used for those methods. Pre-incubation may be carried out by adding the insect cellular membrane expressing human P-glycoprotein to the reaction buffer solution including vanadic acid and [.sup.3H] ATP. Following this, the test compound may be added for reaction, after which reaction liquid may be added to a glass filter of 96-well type, and by using the suction cleaning method, adsorption amount of [.sup.3H] ADP into the membrane-expressing P-glycoprotein may be measured in a single step.
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