Systems, illumination subsystems, and methods for increasing fluorescence emitted by a fluorophore
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United States of America Patent
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Issued Date -
Jun 22, 2006
app pub date -
Dec 16, 2005
filing date -
Dec 17, 2004
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Abandoned
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Abstract
Systems, illumination subsystems, and methods for increasing fluorescence emitted by a fluorophore are provided. One system configured to measure fluorescence of particles includes an illumination subsystem configured to illuminate the particles with light having linear polarization oriented in a non-vertical direction, circular polarization, or elliptical polarization. The polarization of the light causes fluorescence emitted by the fluorophore to be brighter than fluorescence emitted by the fluorophore when illuminated with linearly polarized light oriented in a predominantly vertical direction or non-polarized light. The system also includes a detection subsystem configured to generate output signals responsive to the fluorescence emitted by the fluorophore.
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