Screening to optimize RNAi
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
N/A
Issued Date -
N/A
app pub date -
Dec 7, 2005
filing date -
Aug 30, 2001
priority date (Note) -
Abandoned
status (Latency Note)
Importance
Abstract
Methods and compositions are provided for screening RNAi molecules for efficiency of modulation, particularly inhibition, of expression of genes. The gene for the target protein is fused to a DNA sequence encoding a small fragment of .beta.-galactosidase. The fragment is competent to complex with a large fragment of .beta.-galactosidase to form an active enzyme. By adding to any of the fusion protein expressed the large fragment and a substrate that produces a detectable product, the amount of detectable product produced is related to the efficiency of modulation of expression by the RNAi. The method finds particular application in high throughput screening.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully