Use of caspase enzymes for maturation of engineered recombinant polypeptide fusions
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United States of America Patent
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Issued Date -
May 11, 2006
app pub date -
Feb 23, 2004
filing date -
Feb 21, 2003
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Abandoned
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Abstract
The invention relates to methods of producing and isolating recombinant proteins using fusion protein constructs comprising specific recognition sites for a maturating protease. The invention specifically relates to the use of caspase proteases for the maturation of engineered recombinant fusion proteins or polypeptides. These molecules are engineered using recombinant DNA technology to comprise a specific target sequence for a caspase between a first fusion part and the polypeptide of interest. After processing, the desired mature format of the protein or polypeptide is obtained.
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