Engineered biosynthesis of novel polyenes
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United States of America Patent
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Issued Date -
Apr 6, 2006
app pub date -
May 27, 2002
filing date -
May 31, 2001
priority date (Note) -
Abandoned
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Abstract
The complete DNA sequence of the gene cluster encoding the polypeptides responsible for the biosynthesis of the polyene antibiotic amphotericin of S. nodosus is provided. Engineered alteration of the amphC) gene so as to eliminate production of amphotericin A in favour of the more active form amphotericin B. as well as the production of amphotericin analogues having altered characteristics, is enabled by manipulation of the sequences of the cluster.
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