Method of quantitating proteins and genes in cells using a combination of immunohistochemistry and in situ hybridization
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United States of America Patent
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Issued Date -
Feb 23, 2006
app pub date -
Aug 18, 2004
filing date -
Aug 18, 2004
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Abandoned
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Abstract
The disclosure provides a system for and method of accurately determining the status of a protein target and a nucleic acid target in a biological sample (e.g., a tissue or cell sample). Provided are methods of quantitating a protein target and a nucleic acid target in a tissue or cell sample using image analysis to correlate the optical density of a chromogenic stain to the concentration of a target molecule in a biological sample. The use CISH in combination with IHC to accurately determine the status of two independent target molecules is provided. A computer-aided image analysis system is used to process and analyze optical images of a chromagen-stained tissue or cell sample and to determine the optical density of the stained tissue or cell sample.
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