Nucleic acid sequences having an activity of regulating translation efficiency and utilization thereof
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United States of America Patent
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Issued Date -
Feb 9, 2006
app pub date -
Feb 8, 2005
filing date -
Aug 4, 2004
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Abandoned
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Abstract
The present invention provides a polynucleotide comprising a nucleic acid sequence having an activity of regulating the translation efficiency of a template in a cell-free protein synthesis system and also provides a method for utilizing the same, etc. Protein synthesis is carried out by a translation template containing a polynucleotide comprising a nucleic acid sequence which is to be an object to be selected, a polyribosome fraction is prepared from the reaction solution and a nucleic acid sequence bonding to ribosome is analyzed whereupon a selection is done.
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