Peptide mass spectrometry rich in daughter ions
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United States of America Patent
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Issued Date -
Feb 2, 2006
app pub date -
May 25, 2005
filing date -
May 25, 2004
priority date (Note) -
Abandoned
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Abstract
Methods of analysing peptides by mass spectrometry are disclosed. In particular, methods of analysing peptides by fragmentation mass spectrometry (MS.sup.n, where n is at least 2) are disclosed. The methods involve derivatisation of a peptide at its N-terminus such that peaks corresponding to both a and b (and y) daughter ions are identifiable in fragmentation mass spectra of the derivatised peptide. The fragmentation mass spectra of the derivatised peptide contain additional information (relative to the fragmentation mass spectra of the underivatised peptide) useful for determination of the amino acid sequence of the peptide.
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