Method for identifying protein-protein interactions
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United States of America Patent
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Issued Date -
Jan 26, 2006
app pub date -
May 21, 2003
filing date -
May 22, 2002
priority date (Note) -
Abandoned
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Abstract
The properties of yeast help, a type I ER membrane protein which is involved in the unfolded protein response (UPR), have been exploited to develop a. system for the detection and study of interactions between extracellular and/or membrane proteins. In the system, proteins of interest are fused to the lumenal N-terminus of a truncated Ire1p. A specific interaction between two partners may be visualized through dimerization of the Ire1p moiety which, either, directly or indirectly, results in a detection means, for example, the expression of a selectable reporter gene. Depending on the type of reporter gene used, its expression can positively or negatively influence cell growth, thus allowing selection of both stimulation and inhibition of protein-protein interactions. The system presented here can also be used to study intracellular protein interactions.
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