Differential serological diagnosis of equine infectious anemia virus infected and vaccinated horses using recombinant S2 protein
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
N/A
Issued Date -
N/A
app pub date -
Aug 24, 2005
filing date -
Sep 9, 2000
priority date (Note) -
Abandoned
status (Latency Note)
Importance
Abstract
We describe here the development and optimization of an embodiment of a new serologic EIAV diagnostic ELISA assay to detect serum antibodies to the EIAV S2 protein that are produced in infected horses, but not in horses inoculated with the EIAV.sub.UK.DELTA.S2 vaccine virus. An embodiment of the test S2 protein antigen was developed using the S2 gene sequence from the EIAV.sub.UK strain of virus and a series of modifications to facilitate production and purification of the diagnostic antigen, designated HS2G. Using this embodiment of an HS2G as antigen, we describe the development of an affinity ELISA (NN-ELISA) that provides a sensitive and specific detection of S2-specific serum antibodies in experimentally and field infected horses (22/24), without detectable reactivity with immune serum from uninfected (12/12) or vaccinated (29/29) horses.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully