Refolding of ion channel proteins
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United States of America Patent
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app pub date -
Apr 8, 2005
filing date -
Oct 11, 2002
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Abandoned
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Abstract
A method of ion channels folded into their active structure, or functional subunits thereof, is described. For this purpose, initially expressed subunits, which are solubilized and denatured in a first detergent, of an ion channel are provided, and subsequently the first detergent is replaced by a second detergent which induces folding of the subunits of an ion channel into their native structure. The subunits of the ion channel are then assembled into its active structure.
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