Method of utilizing the 5'end of transcribed nucleic acid regions for cloning and analysis
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United States of America Patent
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Issued Date -
Nov 10, 2005
app pub date -
Jun 9, 2005
filing date -
Jun 12, 2002
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Abstract
A method is disclosed for obtaining the 5'ends of transcribed regions from a plurality of nucleic acid fragments obtained from biological materials or synthetic pools. DNA fragments encoding the 5'ends are enriched for their individual analysis or for the analysis of concatemers thereof. The sequence information derived from 5' ends can be used for characterization and cloning of the transcriptome.
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