Methods and compositions for in vitro amplification of extrachromosomal nucleic acid
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United States of America Patent
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Issued Date -
Nov 3, 2005
app pub date -
Nov 26, 2004
filing date -
Nov 26, 2003
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Abstract
Compositions and methods are provided for the in vitro amplification of nucleic acid and, in particular, for the amplification of extrachromosomal nucleic acid molecules having a molecular weight of one kilobase or greater. The design and use of primers, low ionic strength amplification buffer, low co-solvent containing buffer, and combined polymerization/ligation reaction buffers are described in this regard.
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