Method for amplifying nucleic acid sequence
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United States of America Patent
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Issued Date -
Oct 27, 2005
app pub date -
Oct 27, 2004
filing date -
Mar 19, 1999
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Abandoned
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Abstract
A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
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