Method of DNA shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process
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United States of America Patent
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Issued Date -
Sep 22, 2005
app pub date -
Nov 4, 2004
filing date -
Jul 9, 1996
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Abandoned
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Abstract
Disclosed is a process of performing 'sexual' PCR which includes generating random polynucleotides by interrupting or blocking a synthesis or amplification process to show or halt synthesis or amplification of at least one polynucleotide, optionally amplifying the polynucleotides, and reannealing the polynucleotides to produce random mutant polynucleotides. Also provided are vector and expression vehicles including such mutant polynucleotides, polypeptides expressed by the mutant polynucleotides and a method for producing random mutant polypeptides.
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