Method for the detection of nucleic acid molecules
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United States of America Patent
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Issued Date -
Sep 8, 2005
app pub date -
Jul 12, 2004
filing date -
Mar 2, 2001
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Abandoned
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Abstract
There is provided a method of simultaneously detecting at least two mutually different nucleic acid molecules in a sample, wherein in a first step a multiplex-PCR and in a second step a hybridizing reaction is carried out with probes immobilized on a microarray, whereupon the hybridized PCR products are detected and optionally quantitated, wherein the probes employed for the hybridizing reaction which in each case will hybridize specifically with the mutually different nucleic acid molecules have melting temperatures which differ from each other by 2.degree. C. at the most, preferably by 1.degree. C. at the most.
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