Method for making full-length coding sequence cDNA libraries
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United States of America Patent
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Issued Date -
Aug 11, 2005
app pub date -
Apr 12, 2002
filing date -
Apr 12, 2002
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Abstract
The present invention relates to a method for making cDNA libraries wherein the cDNA inserts comprise the full-length of the coding sequences but having lengths less than the full-length of the mRNA. The method comprises binding a tag molecule to a diol structure present in the 5' Cap sites of mRNAs, forming RNA-DNA hybrids by reverse transcription to synthesize the first cDNA strand, separating RNA-DNA hybrids carrying a DNA corresponding to a full-length of mRNAs from RNA-DNA hybrids formed above by using a function of the tag molecule, and synthesizing the second cDNA strand by self-priming the first cDNA strand. The resulting cDNA libraries do not contain the full-length of the mRNAs but do contain the full-length of the coding sequences of the mRNAs.
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