Method and apparatus for analyzing amino acid, peptide, protein, saccharide or lipid

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United States of America Patent

APP PUB NO 20050170525A1
SERIAL NO

10503349

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Abstract

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The present invention aims to easily and simply perform selective detection/identification, extraction, separation, and purification of a phosphorylated amino acid, phosphorylated peptide, or phosphorylated protein from a sample containing any one of them, comprising the steps of: retaining the phosphorylated amino acid, phosphorylated peptide, or phosphorylated protein in a pretreatment column by passing the sample containing the phosphorylated amino acid, phosphorylated peptide, or phosphorylated protein through a reversed phase HPLC column having a titanium dioxide column as the pretreatment column and retaining a non-phosphorylated amino acid, peptide, or protein in the reverse phase HPLC column; eluting the non-phosphorylated amino acid, peptide, or protein that is retained in the reversed phase HPLC column; eluting the phosphorylated amino acid, phosphorylated peptide, or phosphorylated protein retained in the pretreatment column with buffer; and detecting it.

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Patent Owner(s)

Patent OwnerAddress
GL SCIENCES INCORPORATEDTOKYO 163-1130

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kuroda, Ikuma Saitama-ken, JP 1 3
Nakamura, Hiroshi Chiba, JP 877 11765
Takahashi, Kouji Saitama, JP 124 1629

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