Methods of purification of cytochrome p450 proteins and of their crystallizing
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United States of America Patent
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Issued Date -
Jul 28, 2005
app pub date -
Mar 15, 2005
filing date -
May 30, 2002
priority date (Note) -
Abandoned
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Abstract
The invention provides a method for the purification of cytochrome P450 molecules, the method comprising expressing in a host cell culture a cytochrome P450 molecule; recovering said cells from said culture and suspending said cells in a high salt buffer; lysing said cells and removing cell debris to provide a high-salt lysate; adding to said lysate a detergent to provide a high-salt-detergent lysate; and recovering said P450 from said lysate. The method provides yields of P450 proteins suitable for crystallization.
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