Methods for multiple parameters screening and evolution of cells to produce small molecules with multiple functionalities
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United States of America Patent
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Issued Date -
Jul 28, 2005
app pub date -
Jul 26, 2004
filing date -
Jan 25, 2002
priority date (Note) -
Abandoned
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Abstract
The present invention relates to the field of selecting cells producing compounds that fulfil at least 2 predetermined functionalities. The invention primarily focuses on methods for screening of cells for two or more functionalities related to small molecules generated by host cells with novel gene assemblies. The methods in one aspect of the invention are combined with methods for evolution of cells to acquire at least two predetermined functionalities related to small molecules. Furthermore, the invention relates to methods for generation of lead compounds that are selected for a primary function and at least one further secondary function. The methods according to the invention may in particular be used in high troughput screening.
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