Immunoassays
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United States of America Patent
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Issued Date -
Jul 21, 2005
app pub date -
Dec 2, 2004
filing date -
Sep 30, 2003
priority date (Note) -
Abandoned
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Abstract
This invention provides a method wherein a protein sparingly soluble in water, or a protein in a hardly extractable state, is detected sharply and easily in subsequent immunoreaction while high efficiency of extraction from a sample is maintained. Disclosed is a highly sensitive and easy immunoassay characterized in that a water-sparingly-soluble protein in a sample, or a protein in a hardly extractable state, is extracted/solubilized with an aqueous solvent containing an ionic surfactant at relatively high concentration, and then the protein in the extract is detected directly by an antibody raised against the protein previously denatured with the ionic surfactant.
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