Methods for generating monoclonal antibody against fusion protein containing peptide fragment derived from membrane protein
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United States of America Patent
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Issued Date -
Jun 9, 2005
app pub date -
Nov 12, 2004
filing date -
Feb 8, 2002
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Abandoned
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Abstract
Methods are provided for selecting proteins, in particular antibodies, against a wide variety of membrane proteins. In one aspect, a method is provided for eliciting monoclonal antibody in a non-human animal administered with a target membrane protein by using a fusion protein as an immunogen that contains a peptide fragment derived from the target membrane protein. The fusion protein can also be used as a ligand for screening a library of proteins for protein-ligand binding pairs in vitro by using mRNA, ribosome, phage, or cell display. The selected proteins can be used for therapeutics and diagnostics targeting the membrane proteins.
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