Two-layer antibody capture system
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United States of America Patent
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Issued Date -
Jun 2, 2005
app pub date -
Jul 2, 2004
filing date -
Jul 2, 2003
priority date (Note) -
Abandoned
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Abstract
A multimolecular complex that includes an anti-immunoglobulin (anti-Ig) linking antibody reversibly bound to a substrate, a primary antibody bound to the anti-Ig linking antibody to yield an immobilized primary antibody, and optionally, an antigen is bound to the immobilized primary antibody; and a method for immunochemically immobilizing a molecule of interest, such as an enzyme, using the multimolecular complex. The binding interaction between the substrate and the linking antibody is preferably a biotin/streptavidin interaction.
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