Search for cancer markers by a novel screening method
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United States of America Patent
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Issued Date -
May 19, 2005
app pub date -
Mar 24, 2004
filing date -
Sep 25, 2001
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Abandoned
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Abstract
The present invention relates to a method of concentrating low molecular weight peptides in the supernatant of serum-free cultured cells, said method comprising allowing the peptides to bind to a strong cation exchanger under an acid condition, and eluting them under an alkali condition to concentrate the peptide. Furthermore, peptides having the amino acid sequence as set forth in SEQ ID NO: 1 or 2, and a method of screening cancer markers using antibody to these peptides, are disclosed.
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