Method for labeled amidation of biological molecule and method for analyzing biological molecule
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United States of America Patent
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Issued Date -
Apr 28, 2005
app pub date -
Oct 22, 2004
filing date -
Oct 24, 2003
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Abandoned
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Abstract
The present invention provides a method for improving the sensitivity of mass spectrometry for biological molecules. The present invention also provides a method for a rapid and simple analysis of biological samples by making use of the method for improving the sensitivity of mass spectrometry. A method for labeled amidation, comprising the step of reacting an amidating reagent labeled with .sup.15N, an isotope of nitrogen, with the carboxyl group of a biological molecule. A method for analyzing a biological molecule, comprising the steps of performing amidation to a carboxyl group of a biological molecule to obtain an amidated form of the biological molecule, and subsequently subjecting the amidated form of the biological molecule to mass spectrometry. A labeled amidated form of a biological molecule wherein a carboxyl group of the biological molecule is amidated as .sup.15N-labeled amide group.
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