High-throughput screening assay for inhibitors of replication protein A
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United States of America Patent
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Issued Date -
Apr 21, 2005
app pub date -
Oct 7, 2004
filing date -
Oct 7, 2003
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Abstract
A homogeneous high-throughput assay is provided which rapidly screens compounds to determine degree of inhibition of Replication Protein A (RPA) binding of DNA. Inhibition by a compounds causes a change in the amount of a detectable signal and the degree of inhibition is determined by comparing the amount of detectable signal with an amount of background detectable signal. The assay comprises adding a volume of RPA to a plurality of wells, adding a volume of each of the plurality of compounds to the plurality of wells, adding reporter-labeled DNA to each of the wells, and measuring the detectable signal emitted from each of the wells. The assay is useful in screening large numbers of compounds for potential utility in cancer treatment strategies, particularly in treatment strategies for chemotherapeutically resistant cancers.
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