Transposon-mediated random codon-based mutagenesis
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United States of America Patent
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Issued Date -
Apr 7, 2005
app pub date -
Jul 3, 2003
filing date -
Jul 4, 2002
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Abstract
A method for evolving a polypeptide or a polynucleotide coding therefor, which comprises preparing a library of mutant polynucleotides through transposon-mediated random substitution, insertion or deletion of a multiple of three nucleotides on a polynucleotide coding for a target protein, expressing the mutant polynucleotides in a host cell and screening for a polypeptide having a desired property.
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