Methods for identifying inhibitors of sterol 14-alpha-demethylase
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
N/A
Issued Date -
Apr 7, 2005
app pub date -
Oct 3, 2003
filing date -
Oct 3, 2003
priority date (Note) -
Abandoned
status (Latency Note)
Importance
Abstract
In the methods of the present invention, the change in absorbance of an obtusifoliol 14.alpha.-demethylase (OBT-DM) enzyme upon binding of an inhibitor is used to advantage for the identification of inhibitors of OBT-DM activity. Rather than measuring the absorbance of an OBT-DM/inhibitor complex over a spectrum of wavelengths as described previously, the present invention discloses methods for identifying type II inhibitors of OBT-DM by monitoring the absorbance only at 413 nm and 432 nm. The methods of the invention enable the concurrent testing of multiple compounds using a high throughput format such as with 96- or 384-well plates. The OBT-DM polypeptides of the invention include plant, fungal and human OBT-DM polypeptides, and in particular, Arabidopsis thaliana OBT-DM polypeptide
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully