Protein or polypeptide having lachrymatory factor-producing enzymatic activity, DNA coding for said protein or polypeptide, method for preparation of the protein or polypeptide having lachrymatory factor-producing enzymatic activity by using said DNA and nucleic acid molecule having a function of repressing translation of mRNA with respect to said protein or polypeptide
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United States of America Patent
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app pub date -
Sep 1, 2004
filing date -
Mar 1, 2002
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Abstract
The present invention relates to protein or polypeptide capable of converting 1-propenylsulfenic acid concerning the formation of lachrymatory factors generated when plants such as onions are broken into pieces or cut into lachrymatory factors, and also to DNA (lachrymatory factor-producing enzyme genes) encoding the protein or polypeptide; a primer usable for isolating a gene of a lachrymatory factor-producing enzyme of the genus allium and a method for isolating the gene with the primer; a recombinant vector containing the DNA; a transformant containing the recombinant vector containing the DNA; a method for preparing protein or a polypeptide having lachrymatory factor-producing enzymatic activity by culturing host cells transformed with the recombinant vector containing the DNA; protein or a polypeptide having the lachrymatory factor-producing enzymatic activity; and a nucleic acid molecule having a function of repressing the translation of mRNA on protein or polypeptide having the lachrymatory factor-producing enzymatic activity.
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