Displacement sandwich immuno-PCR
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
N/A
Issued Date -
Feb 3, 2005
app pub date -
Nov 3, 2003
filing date -
Nov 1, 2002
priority date (Note) -
Abandoned
status (Latency Note)
Importance
Abstract
The present invention is directed to labeling antibodies with DNA in a convenient kit format for the purpose of producing and utilizing nucleic acid detection immunoassays. The process comprises treating a reporter antibody with a chemically activated strand of DNA, which serves as a template for any DNA amplification system. The reporter antibody-DNA conjugate is used to form a complex with corresponding antigen. The complex can be detected by the production of multiple copies of the DNA template. The kit may also provide an activated DNA sequence to label a capture antibody. The DNA conjugated to the capture antibody is modified to contain a high affinity binding ligand for the purpose of coating a solid support. The coated solid support functions to capture the immune complex containing reporter antibody in a sandwich assay format. The DNA sequence of the capture antibody may also have properties providing for the detachment of the complex from the solid support.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully