Cryopreservation medium for primate embryo stem cells and cryopreservation method
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United States of America Patent
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Issued Date -
Feb 3, 2005
app pub date -
Jul 30, 2004
filing date -
Jan 31, 2002
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Abandoned
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Abstract
A cryopreservation medium and a cryopreservation method which make it possible to cryopreserve ES cells from primates simply with high viability are provided. A cryopreservation medium containing a cryoprotectant at a Concentration of from 12% (W/V) to 50% (W/V) and a cryopreservation method for primate embryonic stem cells, which comprises a step of suspending primate embryonic stem cells in the cryopreservation medium, and a refrigeration step of freezing the suspension of the primate embryonic stem cells in the cryopreservation medium by cooling it to -80.degree. C. or below at a rate of from 0.5.degree. C. to 10.degree. C. per minute, and a preservation step of storing the frozen suspension of primate embryonic stem cells in the cryopreservation medium enable simple cryopreservation of primate embryonic stem cells with high viability.
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