Methods and compositions for RNA detection and quantitation
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United States of America Patent
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Issued Date -
Dec 23, 2004
app pub date -
Jan 29, 2004
filing date -
Jan 29, 2003
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Abandoned
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Abstract
The present invention generally relates to methods of making cDNA molecules, amplification of RNA by PCR and cDNA libraries. The invention also relates to kits for carrying out the methods of the invention. Methods for improved and more efficient conversion of RNA into cDNA are provided, which in turn can be used in a variety of procedures in molecular analysis of gene expression. The present invention is also directed to compositions comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.
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