Mutant rna polymerases

Number of patents in Portfolio can not be more than 2000

United States of America Patent

APP PUB NO 20040259089A1
SERIAL NO

10480233

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Abstract

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It is intended to provide novel mutant RNA polymerases enabling a transcriptional sequencing method whereby a high SN ratio can be achieved in sequence analysis with the use of capillary and longer and more accurate base sequencial data can be obtained by a single reaction. More specifically, a mutant RNA polymerase derived from a wild type RNA polymerase by substitution of at least one amino acid and deletion of at least one amino acid, or a mutant RNA polymerase derived from a wild type RNA polymerase by deletion of at least one amino acid, wherein the above-described substitution and/or deletion of amino acid(s) have been performed so that the resultant mutant RNA polymerase has an enhanced ability to incorporate 3'-deoxynucleotide as a substrate compared with the wild type RNA polymerase corresponding thereto.

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Patent Owner(s)

Patent OwnerAddress
NIPPON GENETECH CO LTDKONGO-NISHIKI-CHO BLDG 5 KANDA-NISHIKI-CHO 1-CHOME CHIYODA-KU TOKYO 101-0054

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Watahiki, Masanori Toyama-shi, JP 13 34
Yoneda, Yuko Toyama-shi, JP 7 29

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