Methods of derivation and propagation of undifferentiated human embryonic stem (HES) cells on feeder-free matrices and human feeder layers

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United States of America Patent

SERIAL NO

10811694

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Abstract

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The present invention relates to the field of stem cell culture, in particular undifferentiated stem cell culture and to methods for derivation and propagation of such cells. More particularly, the invention relates to derivation and propagation of undifferentiated HES cells on human feeder layers and/or in the absence of a feeder layer. The human feeder layers may be selected from the group including human fetal muscle (HFM), human fetal skin (HFS), human adult fallopian tube (HAFT) fibroblasts and human adult skin cells. They may be cultured in the presence of a suitable medium selected from the group including Human Embryonic Stem Cell (HES), Knockout (KO), or Human Feeder (HF) medium supplemented with or without human serum.

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Patent Owner(s)

Patent OwnerAddress
ES CELL INTERNATIONAL PTE LTD60 BIOPOLIS STREET #01-03 GENOME SINGAPORE 138672

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bongso, Ariffeen Kent Ridge, SG 6 98
Chui-Yee, Fong Kent Ridge, SG 2 10
Richards, Mark Kent Ridge, SG 31 459
Woon-Khiong, Chan Kent Ridge, SG 2 10

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