Heat-labile desoxyribonuclease I variants
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United States of America Patent
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Issued Date -
Dec 9, 2004
app pub date -
Dec 19, 2003
filing date -
Dec 20, 2002
priority date (Note) -
Abandoned
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Abstract
According to the invention, the desoxyribonuclease with increased thermolability is a variant, by way of amino acid substitution, of bovine pancreatic desoxyribonuclease I. The variant protein retains desoxyribonuclease activity. Moreover, the specific desoxyribonuclease activity of the variant of bovine pancreatic desoxyribonuclease I is approximately zero units per mg of protein following heating of the variant of bovine pancreatic desoxyribonuclease I for about 5 min at a temperature below 95.degree. C. In addition, the variant of bovine pancreatic desoxyribonuclease I has no measurable ribonuclease activity.
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