Phosphine-containing formulations for chemiluminescent luciferase assays
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United States of America Patent
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Issued Date -
Nov 4, 2004
app pub date -
Jun 23, 2004
filing date -
Nov 16, 2001
priority date (Note) -
Abandoned
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Abstract
A composition for the chemiluminescent assay of the activity of a luciferase comprising (i) a substrate for the luciferase, which, upon enzymatic reaction with the luciferase in the presence of any required cosubstrate and/or cofactor, yields a detectable chemiluminescent singal, (ii) any cosubstrate and/or cofactor required or beneficial for enzymatic activity of the luciferase, and, as an improvement of the composition, (iii) a water-soluble, organic phosphine-containing compound, which enables the light output from the enzymatic reaction to be modulated. The composition can be used in applications designed to quantitate the presence of the enzyme(s) itself, either in single or dual format, or in applications designed to quantitate the presence of a required cosubstrate, such as ATP.
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