Process for obtaining growth factor (tgf-beta and igf-1), lactoperoxidase and immunoglobulins preparations from milk products having low mutual cross-contamination
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United States of America Patent
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Issued Date -
Nov 4, 2004
app pub date -
Jun 21, 2004
filing date -
Jul 20, 2001
priority date (Note) -
Abandoned
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Abstract
Process for extracting beneficial compounds, in particular growth factors, such as TGF .beta. and IGF-1 from milk. In this process a hydrophobic interaction chromatography step is included. A resin having a butyl group, or a phenyl group as the ligand is used as hydrophobic interaction resin. The resin can be eluted with a salt gradient which, when the ligand is a phenyl group, contains substantially no alcohol, and thus resulting in fractions enriched in the desired growth factors. These fractions can be separated further with a hydroxyapatite column.
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