Cell division-visualized cell and method of production of the same, method of detection of fluorescence, method of evaluation of influence upon cell division, and method of screenig
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United States of America Patent
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Issued Date -
Oct 21, 2004
app pub date -
Nov 24, 2003
filing date -
Dec 9, 2002
priority date (Note) -
Abandoned
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Abstract
The present invention relates to a cell division-visualized cell capable of visualizing cell division through incorporating fluorescent proteins into the cell, and a method of the production of the same, and a method of the visualization of cell division, a method of the evaluation of an influence upon cell division and a method of the screening using the same. In the present invention, state of cell division is observed through visualizing cell division by (1) obtaining a fusion gene by allowing fusion of a gene of a protein that constitutes a cell structure which reflects the situation of cell division and a gene of a fluorescent protein, then (2) introducing three or more kinds of the aforementioned fusion genes of which fluorescent protein being the different kind into a host cell to obtain a cell division-visualized cell, and thereafter (3) allowing expression of the aforementioned fluorescent proteins to detect fluorescence derived from the aforementioned fluorescent proteins during cell division of the cell division-visualized cell in a time dependent manner. Further, by concurrently culturing a subject substance and the cell division-visualized cell of the invention, a subject substance that exerts an influence upon cell division can be selected.
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